Sera of Overweight Patients Alter Adipogenesis and Osteogenesis of Bone Marrow Mesenchymal stromal Cells, a Phenomenon that also Persists in Weight Loss Individuals

Background: Overweight and obesity represents a complex interaction of genetics, diet, metabolism, and physical activity. Abnormal or excessive body fat accumulation increases the risk of pathologies, such as heart disease, diabetes, and high blood pressure. Methods and results: In a previous paper, we investigated the influence of overweight individuals’ sera on in vitro biology of mesenchymal stromal cells, which contain a subpopulation of multipotent stem cells. This report extended the study by evaluating effects of sera collected from weight loss patients on stromal cultures. Results showed that an increase in fat mass induces physiological changes that negatively impact on stromal stem cell adipogenesis and osteogenesis with a bias toward adipocyte differentiation. This phenomenon occurred with sera from overweight patients and persisted also with sera of individuals that underwent loss of weight. Significant changes in obesity related cytokines were detected in sera of overweight and weight loss compared to healthy weight individuals. These changes may explain some of the biological effects detected on stromal stem cell functions. Sera of Overweight Patients Alter Adipogenesis and Osteogenesis of Bone Marrow Mesenchymal Stromal Cells, a Phenomenon that also Persists in Weight Loss Individuals Angela Ferone1, Giovanni Messina2, Nicola Alessio1, Stefania Capasso1, Marilena Cipollaro1, Marcellino Monda2, Gianfranco Peluso3, Umberto Galderisi1 and Giovanni Di Bernardo1* 1Department of Experimental Medicine, Biotechnology, Medical Histology and Molecular Biology Section, Second University of Naples, Naples, Italy 2Department of Experimental Medicine, Human Physiology and Unit Of Dietetic and Sports Medicine Section, Second University of Naples, Naples, Italy 3Institute Bioscience and Bioresources; Cnr; Naples, Italy *Corresponding author: Giovanni Di Bernardo, Department of Experimental Medicine, Biotechnology, Medical Histology and Molecular Biology Section, Second University of Naples, Via S.M. Costantinopoli 16, 80138 Naples, Italy, Tel: +39 0815665838; Fax: +39 0815667547; E-mail: [email protected] Received June 09, 2016; Accepted June 27, 2016; Published July 04, 2016 Citation: Ferone A, Messina G, Alessio N, Capasso S, Cipollaro M, et al. (2016) Sera of Overweight Patients Alter Adipogenesis and Osteogenesis of Bone Marrow Mesenchymal Stromal Cells, a Phenomenon that also Persists in Weight Loss Individuals. J Stem Cell Res Ther 6: 347. doi:10.4172/2157-7633.1000347 Copyright: © 2016 Ferone A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.